Intramolecular platinum migration on a peptide in gas phase during collision-induced dissociation

Zhifeng Du; Yun Qu; Nicholas P Farrell

doi:10.1016/j.jinorgbio.2019.110858

Intramolecular platinum migration on a peptide in gas phase during collision-induced dissociation

J Inorg Biochem. 2020 Jan:202:110858. doi: 10.1016/j.jinorgbio.2019.110858. Epub 2019 Sep 13.

Authors

Zhifeng Du¹, Yun Qu², Nicholas P Farrell³

Affiliations

¹ Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284-2006, USA; Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
² Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284-2006, USA. Electronic address: yqu@vcu.edu.
³ Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284-2006, USA.

PMID: 31689625
DOI: 10.1016/j.jinorgbio.2019.110858

Abstract

We report the migration of platinum ligand unit {Pt(en)}₂ (en = ethylenediamine) on a short peptide during collision-induced dissociation fragmentation combined with the characterization of the same species by 2D [¹H,¹⁵N] HSQC (Heteronuclear Single Quantum Coherence) NMR spectroscopy. The NMR spectrum showed that the cysteine is platinated while the MS/MS (Tandem mass spectrometry) showed the platination at glutamic acid. Our results provide the first experimental evidence of platinum migration on peptide during collision-induced dissociation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nuclear Magnetic Resonance, Biomolecular*
Peptides / chemistry*
Platinum / chemistry*
Tandem Mass Spectrometry*

Substances

Peptides
Platinum