Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.) preservation

Zhixiang Yuan; Yuqi Shi; Fuhong Cai; Jieru Zhao; Qingping Xiong; Yunpeng Wang; Xiaoli Wang; Yonghua Zheng

doi:10.1016/j.foodchem.2019.125604

Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.) preservation

Food Chem. 2020 Mar 30:309:125604. doi: 10.1016/j.foodchem.2019.125604. Epub 2019 Oct 18.

Authors

Zhixiang Yuan¹, Yuqi Shi¹, Fuhong Cai¹, Jieru Zhao¹, Qingping Xiong¹, Yunpeng Wang¹, Xiaoli Wang², Yonghua Zheng³

Affiliations

¹ Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, College of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China.
² Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, College of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China. Electronic address: xlwang@hyit.edu.cn.
³ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

PMID: 31689590
DOI: 10.1016/j.foodchem.2019.125604

Abstract

In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3). The composition of these polysaccharides was determined. PEPS-2 and PEPS-3 showed outstanding antioxidant activities. PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined. Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit. The developed method could be beneficial for improving the shelf-life of strawberries.

Keywords: Exopolysaccharides; Postharvest quality; Pythium arrhenomanes; Strawberry; Structural analysis.

MeSH terms

Anthocyanins / analysis
Ascorbic Acid / analysis
Food Preservation / methods*
Food Storage
Fragaria* / chemistry
Fragaria* / physiology
Fruit* / chemistry
Fruit* / physiology
Malondialdehyde / analysis
Polysaccharides / analysis
Polysaccharides / isolation & purification*
Polysaccharides / pharmacology*
Pythium / chemistry*

Substances

Anthocyanins
Polysaccharides
Malondialdehyde
Ascorbic Acid