Secretory PLA2 specific single domain antibody neutralizes Russell viper venom induced cellular and organismal toxicity

Manpreet Kaur; Abhishek Dubey; Madhu Khatri; Sharvan Sehrawat

doi:10.1016/j.toxicon.2019.10.240

Secretory PLA2 specific single domain antibody neutralizes Russell viper venom induced cellular and organismal toxicity

Toxicon. 2019 Oct 25:172:15-18. doi: 10.1016/j.toxicon.2019.10.240. Epub 2019 Nov 2.

Authors

Manpreet Kaur¹, Abhishek Dubey¹, Madhu Khatri², Sharvan Sehrawat³

Affiliations

¹ Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Sector 81 SAS Nagar Knowledge City, PO Manauli, 140306, Mohali, Punjab, India.
² University Institute of Engineering and Technology, Panjab University Chandigarh, 160014, India.
³ Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Sector 81 SAS Nagar Knowledge City, PO Manauli, 140306, Mohali, Punjab, India. Electronic address: sharvan@iisermohali.ac.in.

PMID: 31689425
DOI: 10.1016/j.toxicon.2019.10.240

Abstract

Despite continued destruction of human lives by snakebites, appreciable improvements in immunotherapies have not been made. We selected and characterized venom-specific single domain antibodies (sdAbs) from a constructed phage display library of camelid variable region of heavy chain of the heavy chain antibodies (V_HHs). Secretory phospholipase A2-specific sdAbs neutralized venom-induced toxicity in vitro and in vivo. Such monoclonal sdAbs could serve as an alternative to help manage snakebites to save lives.

Keywords: Monoclonal Ab; Phage display; RVV envenoming; V(H)H; sdAb.

MeSH terms

Animals
Antibodies, Monoclonal / immunology
Camelids, New World / immunology
Daboia
Humans
Jurkat Cells
Phospholipases A2 / metabolism*
Single-Domain Antibodies / chemistry*
Viper Venoms / chemistry
Viper Venoms / enzymology*
Viper Venoms / toxicity
Zebrafish

Substances

Antibodies, Monoclonal
Single-Domain Antibodies
Viper Venoms
Phospholipases A2