Nanomolar detection of adenosine triphosphate (ATP) using a nanostructured fluorescent chemosensing ensemble

Ling-Xi Huang; Qing Guo; Yong Chen; Peter Verwilst; Subin Son; Jia-Bin Wu; Qian-Yong Cao; Jong Seung Kim

doi:10.1039/c9cc08054f

Nanomolar detection of adenosine triphosphate (ATP) using a nanostructured fluorescent chemosensing ensemble

Chem Commun (Camb). 2019 Dec 7;55(94):14135-14138. doi: 10.1039/c9cc08054f. Epub 2019 Nov 5.

Authors

Ling-Xi Huang¹, Qing Guo¹, Yong Chen², Peter Verwilst³, Subin Son³, Jia-Bin Wu¹, Qian-Yong Cao¹, Jong Seung Kim³

Affiliations

¹ Department of Chemistry, Nanchang University, Nanchang 330031, P. R. China. cqyong@ncu.edu.cn.
² Institute for Advanced Study, Nanchang University, Nanchang 330031, China.
³ Department of Chemistry, Korea University, Seoul 02841, Korea. jongskim@korea.ac.kr.

PMID: 31687696
DOI: 10.1039/c9cc08054f

Abstract

We report a novel nanostructured chemosensing ensemble PyNp-C13/UD, obtained by self-assembling uranine dye (UD) and an amphiphilic pyridinium salt PyNp-C13. The ensemble was developed for the fluorescence turn-on sensing of ATP in aqueous solutions and inside living cells. The assembly operates via an indicator displacement assay (IDA) method with an ultra-low detection limit of 6.8 nM.

MeSH terms

Adenosine Triphosphate / analysis*
Biosensing Techniques
Fluorescein / chemical synthesis
Fluorescein / chemistry*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Nanostructures / chemistry*
Pyridinium Compounds / chemistry
Salts / chemistry
Spectrometry, Fluorescence
Surface-Active Agents / chemistry

Substances

Fluorescent Dyes
Pyridinium Compounds
Salts
Surface-Active Agents
Adenosine Triphosphate
Fluorescein