Proteomic dataset: Profiling of membrane fraction of Escherichia coli isolated from Crohn's disease patients after adhesion and invasion experiments

Olga Bukato; Olga Pobeguts; Daria Rakitina; Julia Baikova; Ivan Butenko; Artemy Silantyev; Gleb Fisunov; Vadim Govorun

doi:10.1016/j.dib.2019.104417

Proteomic dataset: Profiling of membrane fraction of Escherichia coli isolated from Crohn's disease patients after adhesion and invasion experiments

Data Brief. 2019 Aug 20:27:104417. doi: 10.1016/j.dib.2019.104417. eCollection 2019 Dec.

Authors

Olga Bukato¹, Olga Pobeguts¹, Daria Rakitina¹, Julia Baikova¹, Ivan Butenko¹, Artemy Silantyev², Gleb Fisunov¹, Vadim Govorun¹

Affiliations

¹ Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Russian Federation.
² V. P. Serbsky Federal Medical Research Center of Psychiatry and Narcology National Scientific Research Centre on Addictions, Russian Federation.

Abstract

Crohn's disease (CD) is a type of inflammatory bowel disease (IDB). The endoscopic picture of Crohn's disease includes thickened submucosa, transmural inflammation, fissuring ulceration, and non-caseating granulomas. Intestinal microbiome dysbiosis has been described systematically in patients with IBD. In recent decades it was detailed that Escherichia coli, especially adherent-invasive E. coli (AIEC) pathotype, has been implicated in the pathogenesis of IBD, including Crohn's disease (Palmela, et al., 2018). In comparison with commensal strains of E. coli, AIEC strains have a large adhesive-invasive potential therefore its surface composition is of great interest. We presented a dataset of the membrane proteins of strains isolated from patients with Crohn's disease. From the set of Escherichia coli isolated from Crohn's disease patients [2] we chose three isolates with strongest AIEC pathotype. We performed proteome-wide LC-MS analysis of membrane fraction of this isolates after invasion or adhesion-invasion to human intestinal CaCo-2 cell line and prior to this (control). The data including LC-MS/MS raw files and exported MaxQuant search results with fasta files were deposited to the PRIDE repository project accession PXD014250.

Keywords: AIEC; Crohn's disease; Escherichia coli; LC-MS/MS; Membrane proteins; Proteome.