Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes

Rupendra Shrestha; Yao-Tseng Wen; Rong-Kung Tsai

doi:10.1016/j.scr.2019.101590

Generation of hiPSC line TCIERi001-A from normal human epidermal keratinocytes

Stem Cell Res. 2019 Dec:41:101590. doi: 10.1016/j.scr.2019.101590. Epub 2019 Oct 15.

Authors

Rupendra Shrestha¹, Yao-Tseng Wen², Rong-Kung Tsai³

Affiliations

¹ Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan; Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan.
² Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan.
³ Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan; Institute of Eye Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan. Electronic address: rktsai@tzuchi.com.tw.

PMID: 31683100
DOI: 10.1016/j.scr.2019.101590

Abstract

Human induced pluripotent stem cell (hiPSC) line TCIERi001-A was generated from normal human epidermal keratinocytes (NHEK) primary cell line with the nonintegrating system using Sendai reprogramming kit. Sendai particles were used to deliver the defined transcription factors that included three vector preparations, such as polycistronic KLF4-OCT3/4-SOX2, cMYC, and KLF4.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Line
Cellular Reprogramming Techniques*
Epidermis / metabolism*
Female
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Keratinocytes / cytology
Keratinocytes / metabolism*
Kruppel-Like Factor 4