Prokaryotic Genome Expansion Is Facilitated by Phages and Plasmids but Impaired by CRISPR

Na L Gao; Jingchao Chen; Teng Wang; Martin J Lercher; Wei-Hua Chen

doi:10.3389/fmicb.2019.02254

Prokaryotic Genome Expansion Is Facilitated by Phages and Plasmids but Impaired by CRISPR

Front Microbiol. 2019 Oct 16:10:2254. doi: 10.3389/fmicb.2019.02254. eCollection 2019.

Authors

Na L Gao^{1

2}, Jingchao Chen³, Teng Wang¹, Martin J Lercher², Wei-Hua Chen^{1

3

4}

Affiliations

¹ Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular-Imaging, Department of Bioinformatics and Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
² Institute for Computer Science and Department of Biology, Heinrich Heine University, Duesseldorf, Germany.
³ College of Life Science, Henan Normal University, Xinxiang, China.
⁴ Huazhong University of Science and Technology Ezhou Industrial Technology Research Institute, Ezhou, China.

Abstract

Viruses and plasmids can introduce novel DNA into bacterial cells, thereby creating an opportunity for genome expansion; conversely, CRISPR, the prokaryotic adaptive immune system, which targets and eliminates foreign DNAs, may impair genome expansions. Recent studies presented conflicting results over the impact of CRISPR on genome expansion. In this study, we constructed a comprehensive dataset of prokaryotic genomes and identified their associations with viruses and plasmids. We found that genomes associated with viruses and/or plasmids were significantly larger than those without, indicating that both viruses and plasmids contribute to genome expansion. Genomes were increasingly larger with increasing numbers of associated viruses or plasmids. Conversely, genomes with CRISPR systems were significantly smaller than those without, indicating that CRISPR has a negative impact on genome size. These results confirmed that on evolutionary timescales, viruses and plasmids facilitate genome expansion, while CRISPR impairs such a process in prokaryotes. Furthermore, our results also revealed that CRISPR systems show a preference for targeting viruses over plasmids.

Keywords: CRISPR; horizontal gene transfer; plasmids; prokaryotic genome expansion; viruses.