Enhancement of the denitrification in low C/N condition and its mechanism by a novel isolated Comamonas sp. YSF15

Jun Feng Su; Shu Yang; Ting Lin Huang; Min Li; Jia Ran Liu; Yi Xin Yao

doi:10.1016/j.envpol.2019.113294

Enhancement of the denitrification in low C/N condition and its mechanism by a novel isolated Comamonas sp. YSF15

Environ Pollut. 2020 Jan:256:113294. doi: 10.1016/j.envpol.2019.113294. Epub 2019 Sep 24.

Authors

Jun Feng Su¹, Shu Yang², Ting Lin Huang², Min Li², Jia Ran Liu², Yi Xin Yao²

Affiliations

¹ Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China. Electronic address: ys13720769969@sina.com.
² Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.

PMID: 31679877
DOI: 10.1016/j.envpol.2019.113294

Abstract

A novel denitrifying bacterium YSF15 was isolated from the Lijiahe Reservoir in Xi'an and identified as Comamonas sp. It exhibited excellent nitrogen removal ability under low C/N conditions (C/N = 2.5) and 94.01% of nitrate was removed in 18 h, with no accumulation of nitrite. PCR amplification and nitrogen balance experiments were carried out, showing that 68.92% of initial nitrogen was removed as gas products and the nitrogen removal path was determined to be NO₃^--N→NO₂^--N→NO→N₂O→N₂. Scanning electron microscopy and three-dimensional fluorescence spectroscopy were used to track extracellular polymeric substances (EPS). The results show that complete-denitrification under low C/N conditions is associated with EPS, which may provide a reserve carbon source in extreme environments. These findings reveal that Comamonas sp. YSF15 can provide novel basic materials and a theoretical basis for wastewater bioremediation under low C/N conditions.

Keywords: Denitrification; Extracellular polymeric substance (EPS); Low C/N; Metabolic characteristics; Nitrogen balance.

MeSH terms

Aerobiosis
Biodegradation, Environmental
Carbon / analysis*
Comamonas / growth & development*
Comamonas / isolation & purification
Comamonas / metabolism
Denitrification
Extracellular Polymeric Substance Matrix / metabolism
Nitrates / analysis*
Nitrites / analysis*
Nitrogen / analysis*
Wastewater / chemistry
Wastewater / microbiology

Substances

Nitrates
Nitrites
Waste Water
Carbon
Nitrogen