Strand Displacement Strategies for Biosensor Applications

Yifan Dai; Ariel Furst; Chung Chiun Liu

doi:10.1016/j.tibtech.2019.10.001

Strand Displacement Strategies for Biosensor Applications

Trends Biotechnol. 2019 Dec;37(12):1367-1382. doi: 10.1016/j.tibtech.2019.10.001. Epub 2019 Nov 1.

Authors

Yifan Dai¹, Ariel Furst², Chung Chiun Liu³

Affiliations

¹ Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH 44106, USA; Electronics Design Center, Case Western Reserve University, Cleveland, OH 44106, USA. Electronic address: yxd176@case.edu.
² Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Electronic address: afurst@mit.edu.
³ Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH 44106, USA; Electronics Design Center, Case Western Reserve University, Cleveland, OH 44106, USA.

PMID: 31679827
DOI: 10.1016/j.tibtech.2019.10.001

Abstract

DNA has many unique properties beyond encoding genetic information, one of which is its physicochemical stability based on Watson-Crick base pairing. Differences in sequence complementarity between multiple DNA strands can lead to the strand displacement reaction (SDR). SDRs have been regularly applied in synthetic biology, drug delivery, and, importantly, biosensing. SDR-based biosensors have high controllability, high sensitivity, and low interference, and can be used for multiplexed detection. Such biosensors have been demonstrated to detect nearly every class of biomolecule. As the field continues to mature, such platforms can be used as an integral tool for the manipulation of biomolecular reactions, bringing biosensors one step closer to the ultimate goal of point-of-care systems.

Keywords: DNA biotechnology; biosensing strategy; biosensor; strand displacement reaction.

Publication types

Review

MeSH terms

Biosensing Techniques*
DNA / chemistry*

Substances

DNA