Mass spectrometry-based qualitative and quantitative N-glycomics: An update of 2017-2018

Kaijie Xiao; Yuyin Han; Hailun Yang; Haoran Lu; Zhixin Tian

doi:10.1016/j.aca.2019.10.007

Mass spectrometry-based qualitative and quantitative N-glycomics: An update of 2017-2018

Anal Chim Acta. 2019 Dec 24:1091:1-22. doi: 10.1016/j.aca.2019.10.007. Epub 2019 Oct 14.

Authors

Kaijie Xiao¹, Yuyin Han¹, Hailun Yang¹, Haoran Lu¹, Zhixin Tian²

Affiliations

¹ School of Chemical Science & Engineering and Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai, 200092, China.
² School of Chemical Science & Engineering and Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University, Shanghai, 200092, China. Electronic address: zhixintian@tongji.edu.cn.

PMID: 31679562
DOI: 10.1016/j.aca.2019.10.007

Abstract

N-glycosylation is one of the most frequently occurring protein post-translational modifications (PTMs) with broad cellular, physiological and pathological relevance. Mass spectrometry-based N-glycomics has become the state-of-the-art instrumental analytical pipeline for sensitive, high-throughput and comprehensive characterization of N-glycans and N-glycomes. Improvement and new development of methods in N-glycan release, enrichment, derivatization, isotopic labeling, separation, ionization, MS, tandem MS and informatics accompany side-by-side wider and deeper application. This review provides a comprehensive update of mass spectrometry-based qualitative and quantitative N-glycomics in the years of 2017-2018.

Keywords: Differential expression; Mass spectrometry-based qualitative and quantitative N-Glycomics; Monosaccharide composition; N-Glycan; N-Glycosylation; Topological sequence and linkage.

Publication types

Review

MeSH terms

Animals
Carbohydrate Sequence
Cell Line
Glycomics / methods*
Humans
Influenza A Virus, H9N2 Subtype / chemistry
Polysaccharides / analysis*
Tandem Mass Spectrometry / methods*

Substances

Polysaccharides