Oxidative stress responses of golden and brown noble scallops Chlamys nobilis to acute cold stress

Abstract

The noble scallop Chlamys nobilis is an important edible marine bivalve that is widely cultivated in the sea of southern China. Unfortunately, the mass mortality of noble scallops frequently occurs during the winter months. The present study investigated the effects of acute cold stress (8 °C) to the physiological responses of polymorphic noble scallops, by assessing the HSP70 gene expression, total carotenoid content (TCC), total antioxidant capacity (TAC), malondialdehyde (MDA) content, catalase (CAT) activity and superoxide dismutase (SOD) enzymatic activity in different tissues of golden and brown scallops. The results of the present study revealed that MDA, TCC and CAT increased drastically in most tissues in the early stage of acute cold stress (0-3 h), but TCC, SOD and CAT generally showed a downward trend. Within 3-6 h of acute cold stress, MDA content decreased in most tissues and the SOD content increased significantly in most tissues, while TCC and CAT remained at peak. After 6 h of acute cold stress, MDA content continued to increase in most tissues, while TCC, CAT, SOD and TAC decreased or remained at a lower level. For HSP70 expression, up-regulation of the HSP70 gene was observed only in mantle of brown scallops and hemolymph of golden scallops at 3 h and 24 h, respectively. The findings of the present study can better understand the physiological response of noble scallops to acute cold stress.

Keywords: Acute cold stress; Antioxidant enzymes; Carotenoids; Chlamys nobilis; HSP70.