Functional relationship between CgMyD88-1 and CgMyD88-2 in the Pacific oyster

Fish Shellfish Immunol. 2020 Jan:96:138-140. doi: 10.1016/j.fsi.2019.10.060. Epub 2019 Oct 30.

Abstract

MyD88 is a universal adapter protein for the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) signaling pathway. Since invertebrates are believed to lack MyD88-independent pathways, MyD88 appears more critical in oyster TLR signaling pathway. In the Pacific oyster (Crassostrea gigas), two complete paralogues, named as CgMyD88-1 and CgMyD88-2, have been identified. In the current study, we indicated that CgMyD88-1 and CgMyD88-2 might act synergistically to increase the efficiency of immune signaling by activating NF-κB transcription factor. However, we found that upon stimulation with lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid [poly (I:C)], CgMyD88-1 and CgMyD88-2 show differences in their response: CgMyD88-1 accumulated as large spots in the cytoplasm, while CgMyD88-2 assembled in the cytoplasm and in the membrane. Our results support the theory that expansion of these immune genes is associated with functional diversity.

Keywords: Crassostrea gigas; Functional diversity; Innate immunity; MyD88.

MeSH terms

  • Animals
  • Crassostrea / genetics*
  • Crassostrea / immunology
  • Immunity, Innate / genetics*
  • Lipopolysaccharides / pharmacology
  • Myeloid Differentiation Factor 88 / immunology*
  • Poly I-C / pharmacology
  • Signal Transduction / immunology*
  • Toll-Like Receptors / immunology*

Substances

  • Lipopolysaccharides
  • Myeloid Differentiation Factor 88
  • Toll-Like Receptors
  • Poly I-C