Derivation of a MUSIi012-A iPSCs from mobilized peripheral blood stem cells

Chuti Laowtammathron; Pimonwan Srisook; Pimjai Chingsuwanrote; Nittaya Jiamvoraphong; Supaporn Waeteekul; Papussorn Terbto; Yaowalak U-Pratya; Chanchao Lorthongpanich; Surapol Issaragrisil

doi:10.1016/j.scr.2019.101597

Derivation of a MUSIi012-A iPSCs from mobilized peripheral blood stem cells

Stem Cell Res. 2019 Dec:41:101597. doi: 10.1016/j.scr.2019.101597. Epub 2019 Oct 19.

Authors

Chuti Laowtammathron¹, Pimonwan Srisook¹, Pimjai Chingsuwanrote¹, Nittaya Jiamvoraphong¹, Supaporn Waeteekul², Papussorn Terbto³, Yaowalak U-Pratya⁴, Chanchao Lorthongpanich⁵, Surapol Issaragrisil⁶

Affiliations

¹ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand.
² Division of Medical Genetics, Department of Obstetrics & Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
³ Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁴ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
⁵ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand. Electronic address: chanchao.lor@mahidol.ac.th.
⁶ Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Bangkok Hematology Center, Wattanosoth Hospital, BDMS Center of Excellence for Cancer, Bangkok, Thailand.

PMID: 31669974
DOI: 10.1016/j.scr.2019.101597

Abstract

CD34⁺ cells were isolated from mobilized peripheral blood of a healthy donor and reprogrammed by nucleofection with episomal plasmids carrying l-MYC, LIN28, OCT4, SOX2, KLF4, EBNA-1, and shRNA against p53. The obtained MUSIi012-A cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers, and a normal karyotype.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation*
Cells, Cultured
Cellular Reprogramming*
Female
Humans
Kruppel-Like Factor 4
Peripheral Blood Stem Cells / cytology*
Plasmids
Teratoma / pathology*