Investigation of the thermodynamic drivers of the interaction between the high mobility group box domain of Sox2 and bacterial lipopolysaccharide

Biochim Biophys Acta Biomembr. 2020 Feb 1;1862(2):183106. doi: 10.1016/j.bbamem.2019.183106. Epub 2019 Oct 26.

Abstract

Gastric cancer is associated with high mortality and is preceded by an infection with Helicobacter pylori (H. pylori). H. pylori stimulates inflammation which involves the activation of Toll-like receptor 4 by lipopolysaccharide molecules from the H. pylori. This leads to chronic inflammation that can eventually lead to gastric cancer. Sox2 is a member of the high mobility group (HMG) box family of proteins, and recent studies have shown that HMG box proteins can modulate immune response by altering signaling to Toll-like receptors. Sox2 is overexpressed in most types of cancer with the exception of gastric cancer where expression of Sox2 is decreased. Here, we demonstrate that Sox2 can bind LPS and we investigated the thermodynamic drivers of the Sox2/LPS interaction.

Keywords: Gastric cancer; High-mobility group box; Isothermal titration calorimetry; Lipopolysaccharide; Sox2; Tryptophan fluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • HMG-Box Domains*
  • Helicobacter pylori / chemistry
  • Humans
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / metabolism
  • Molecular Docking Simulation*
  • Protein Binding
  • SOXB1 Transcription Factors / chemistry*
  • SOXB1 Transcription Factors / metabolism

Substances

  • Lipopolysaccharides
  • SOXB1 Transcription Factors