Tagged cine magnetic resonance imaging to quantify regional mechanical changes after acute myocardial infarction

Abstract

Purpose: The conventional volumetric approaches of measuring cardiac function are load-dependent, and are not able to discriminate functional changes in the infarct, transition and remote myocardium. We examined phase-dependent regional mechanical changes in the infarct, transition and remote regions after acute myocardial infarction (MI) in a preclinical mouse model using cardiovascular magnetic resonance imaging (CMR).

Methods: We induced acute MI in six mice with left anterior descending coronary artery ligation. We then examined cardiac (infarct, transition and remote-zone) morphology and function utilizing 9.4 T high field CMR before and 2 weeks after the induction of acute MI. Myocardial scar tissue was evaluated by using CMR with late gadolinium enhancement (LGE). After determining global function through volumetric analysis, regional wall motion was evaluated by measuring wall thickening and radial velocities. Strain rate imaging was performed to assess circumferential contraction and relaxation at the myocardium, endocardium, and epicardium.

Results: There was abnormal LGE in the anterior walls after acute MI suggesting a successful MI procedure. The transition zone consisted of a mixed signal intensity, while the remote zone contained viable myocardium. As expected, the infarct zone had demonstrated severely decreased myocardial velocities and strain rates, suggesting reduced contraction and relaxation function. Compared to pre-infarct baseline, systolic and diastolic velocities (v_S and v_D) were significantly reduced at the transition zone (v_S: -1.86 ± 0.16 cm/s vs -0.68 ± 0.13 cm/s, P < 0.001; v_D: 1.86 ± 0.17 cm/s vs 0.53 ± 0.06 cm/s, P < 0.001) and remote zone (v_S: -1.86 ± 0.16 cm/s vs -0.65 ± 0.12 cm/s, P < 0.001; v_D: 1.86 ± 0.16 cm/s vs 0.51 ± 0.04 cm/s, P < 0.001). Myocardial peak systolic and diastolic strain rates (SR_S and SR_D) were significantly lower in the transition zone (SR_S: -4.2 ± 0.3 s^-1 vs -1.3 ± 0.2 s^-1, P < 0.001; SR_D: 3.9 ± 0.3 s^-1 vs 1.3 ± 0.2 s^-1, P < 0.001) and remote zone (SR_S: -3.8 ± 0.3 s^-1 vs -1.4 ± 0.3 s^-1, P < 0.001; SR_D: 3.5 ± 0.2 s^-1 vs 1.5 ± 0.4 s^-1, P = 0.006). Endocardial and epicardial SR_S and SR_D were similarly reduced in the transition and remote zones compared to baseline.

Conclusions: This study, for the first time, utilized state-of-the art high-field CMR algorithms in a preclinical mouse model for a comprehensive and controlled evaluation of the regional mechanical changes in the transition and remote zones, after acute MI. Our data demonstrate that CMR can quantitatively monitor dynamic post-MI remodeling in the transition and remote zones, thereby serving as a gold standard tool for therapeutic surveillance.

Keywords: Cardiovascular magnetic resonance imaging; Left ventricular remodeling; Myocardial infarction; Strain imaging.