Modulation of TLR2 and TLR4 in Macrophages Following Trichinella Spiralis Infection

C Han; J Xu; C H Liu; X Li; P Zhai; A Hashan; M Song

doi:10.2478/helm-2018-0015

Modulation of TLR2 and TLR4 in Macrophages Following Trichinella Spiralis Infection

Helminthologia. 2018 Jul 28;55(3):195-203. doi: 10.2478/helm-2018-0015. eCollection 2018 Sep.

Authors

C Han¹, J Xu¹, C H Liu¹, X Li¹, P Zhai¹, A Hashan², M Song¹

Affiliations

¹ College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China.
² Tiemaike Animal Husbandry and Veterinary station, Fuyun, Xinjiang 836302, PR China.

Abstract

Parasitic helminthes can suppress and/or regulate the host immune response to allow long-term survival and chronic infection where toll-like receptors (TLRs) expressed on macrophages play essential roles in response to parasitic infection. Semi-quantitative PCR and flow cytometry studies about the modulation of TLRs and cytokine profiles in macrophages following T. spiralis infection were performed. TLRs, MyD88 and NF-κB were up-regulated by T. spiralis infection and essential to the parasite life cycles. Cytokines profiles (IL-6, IL-10, IL-12, TNF-α) were modulated during T. spiralis infection. Results suggest that T. spiralis infection may regulate the expression of TLR4 on macrophages and TLR4/MyD88/NF-κB signaling pathways. This study provides further insights into the mechanisms of TLR-mediated post-inflammatory response during T. spiralis infection.

Keywords: Helminth; Immune modulation; Toll-like receptor; Trichinella spiralis.