DNA Replication Inhibitor Geminin and Retinoic Acid Signaling Participate in Complex Interactions Associated With Pluripotency

Spyridon Champeris Tsaniras; George J Delinasios; Michalis Petropoulos; Andreas Panagopoulos; Athanasios K Anagnostopoulos; Maria Villiou; Dimitrios Vlachakis; Vasiliki Bravou; Georgios T Stathopoulos; Stavros Taraviras

doi:10.21873/cgp.20162

DNA Replication Inhibitor Geminin and Retinoic Acid Signaling Participate in Complex Interactions Associated With Pluripotency

Cancer Genomics Proteomics. 2019 Nov-Dec;16(6):593-601. doi: 10.21873/cgp.20162.

Affiliations

¹ Department of Physiology, Medical School, University of Patras, Patras, Greece.
² Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, U.S.A.
³ International Institute of Anticancer Research, Kapandriti, Greece.
⁴ Department of Biology, School of Medicine, University of Patras, Patras, Greece.
⁵ Proteomics Research Unit, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
⁶ Bioinformatics & Medical Informatics Laboratory, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
⁷ Department of Anatomy-Histology-Embryology, Faculty of Medicine, University of Patras, Patras, Greece.
⁸ Laboratory for Molecular Respiratory Carcinogenesis, Department of Physiology, Faculty of Medicine, University of Patras, Patras, Greece.
⁹ Department of Physiology, Medical School, University of Patras, Patras, Greece taraviras@med.upatras.gr.

Abstract

Background/aim: Several links between DNA replication, pluripotency and development have been recently identified. The involvement of miRNA in the regulation of cell cycle events and pluripotency factors has also gained attention.

Materials and methods: In the present study, we used the g:Profiler platform to analyze transcription factor binding sites, miRNA networks and protein-protein interactions to identify novel links among the aforementioned processes.

Results and conclusion: A complex circuitry between retinoic acid signaling, SWI/SNF components, pluripotency factors including Oct4, Sox2 and Nanog and cell cycle regulators was identified. It is suggested that the DNA replication inhibitor geminin plays a central role in this circuitry.

Keywords: DNA replication; GABA; H2AX; Oct4; geminin; mir-452; pluripotency; retinoic acid.

MeSH terms

Cell Cycle Proteins / metabolism
Databases, Genetic*
Geminin / metabolism*
Humans
MicroRNAs / metabolism
Pluripotent Stem Cells / metabolism*
Signal Transduction / drug effects*
Tretinoin / pharmacology*

Substances

Cell Cycle Proteins
GMNN protein, human
Geminin
MicroRNAs
Tretinoin