Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein

Payuda Hansoongnern; Challika Kaewborisuth; Porntippa Lekcharoensuk

doi:10.1016/j.dib.2019.104603

Data on production of mammalian stable cells expressing secretory BEFV transmembrane deleted G protein

Data Brief. 2019 Oct 3:27:104603. doi: 10.1016/j.dib.2019.104603. eCollection 2019 Dec.

Authors

Payuda Hansoongnern¹, Challika Kaewborisuth^{2

3}, Porntippa Lekcharoensuk^{1

2

4}

Affiliations

¹ Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok, 10900, Thailand.
² Center for Advanced Studies in Agriculture and Food (CASAF), KU Institute of Advanced Studies, Kasetsart University, Bangkok, 10900, Thailand.
³ Virology and Cell Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
⁴ Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.

Abstract

Generation of stable cell lines is a widely used technique for continuous recombinant protein production. Advantages of the constitutive stable over the transient protein expression are uniformity of the expression across cell populations as well as high quantity and consistency of the protein yields. This data describe step-by-step procedure for the production of glycoprotein without a transmembrane domain (GΔTM) of bovine ephemeral fever virus (BEFV) by mammalian stable cells. LentiX-293T cells were transfected with four plasmid constructs to generate a recombinant lentivirus. Subsequently, 293T cells were transduced by the recombinant virus and the polyclonal stable cell pools were then selected by puromycin. Next, limiting dilution was performed from each cell pool to isolate the monoclonal stable cells expressing GΔTM protein. Western blot analysis showed that all monoclonal cell clones could stably express GΔTM protein. The data confirms that the stable 293T cell line expressing the secretory GΔTM protein is an attractive platform for antigen production.

Keywords: Bovine ephemeral fever virus; Mammalian stable cell; Protein expression; Transmembrane deleted G protein.