2-Amino-4-(1-piperidine) pyridine exhibits inhibitory effect on colon cancer through suppression of FOXA2 expression

Jieshu Wang; Bo Li; Kun Zhao; Xinyou Su

doi:10.1007/s13205-019-1915-1

2-Amino-4-(1-piperidine) pyridine exhibits inhibitory effect on colon cancer through suppression of FOXA2 expression

3 Biotech. 2019 Nov;9(11):384. doi: 10.1007/s13205-019-1915-1. Epub 2019 Oct 4.

Authors

Jieshu Wang¹, Bo Li¹, Kun Zhao¹, Xinyou Su²

Affiliations

¹ Department of Gastroenterology, Binzhou Center Hospital, Binzhou, 251700 Shandong People's Republic of China.
² 2Department of Oncology, Jinan Central Hospital, No. 105, Jiefang Road, Lixia District, Jinan, 250013 Shandong People's Republic of China.

Abstract

The present study was aimed to investigate the effect of 2-amino-4-(1-piperidine) pyridine on migration and invasion of colon cancer cells. Treatment of colon cancer cells with 2-amino-4-(1-piperidine) pyridine reduced viability in concentration-based manner. The migration potential of HCT116 and HT29 cells was also suppressed on treatment with 2-amino-4-(1-piperidine) pyridine. In HCT116 and HT29 cells, apoptotic cell proportion was increased significantly by 2-amino-4-(1-piperidine) pyridine treatment. The expression of EMT and Vimentin in HCT116 and HT29 cells was reduced markedly on treatment with 2-amino-4-(1-piperidine) pyridine. The expression of E-cadherin was increased in HCT116 and HT29 cells by 2-amino-4-(1-piperidine) pyridine treatment. Treatment with 2-amino-4-(1-piperidine) pyridine reduced the expression of FOXA2 in HCT116 and HT29 cells. The 2-amino-4-(1-piperidine) pyridine treatment reduced growth of tumor in vivo in mice model. In summary, 2-amino-4-(1-piperidine) pyridine treatment inhibits colon cancer cell proliferation through down-regulation of FOXA2 expression. Therefore, 2-amino-4-(1-piperidine) pyridine can be used for the treatment of colon cancer.

Keywords: Forkhead box; Malignant; Mesenchymal transition; Metastasis.