Fermentation slurry from food waste (FSFW) produced at different temperatures (20, 37, and 55 °C) was utilized as external carbon source for promoting nitrogen removal in this study. It was found that high temperatures improved the hydrolysis rate by promoting the hydrolytic enzyme activity. Mesophilic temperature (37 °C) was favorable for organic acid (especially lactic acid) production by selectively enriching the Lactobacillus (with a relative abundance of 90.6%), while thermophilic temperature (55 °C) would restrict the acidogenesis rate (18.9%) and result in the accumulation of carbohydrate in the fermented slurry. Organic acids in the FSFW act as easily biodegradable carbon sources, but the macromolecular and particulate organic components can be utilized as slowly biodegradable carbon sources in the denitrification processes. Using the FSFW as carbon sources to enhance nitrogen removal from wastewater in sequencing batch reactors (SBRs) for more than 150 days, the FSFW produced at thermophilic temperature could significantly promote the microbial metabolic capacity of the activated sludge and improve the nitrogen and phosphate removal efficiencies.
Keywords: Denitrification; Fermentation slurry from food waste (FSFW); Metabolic capacity; Microbial community; Organic acid.