Development, chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction methodology for multi-mycotoxins in maize

Sefater Gbashi; Patrick Berka Njobeh; Sarah De Saeger; Marthe De Boevre; Ntakadzeni Edwin Madala

doi:10.1016/j.foodchem.2019.125526

Development, chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction methodology for multi-mycotoxins in maize

Food Chem. 2020 Mar 1:307:125526. doi: 10.1016/j.foodchem.2019.125526. Epub 2019 Sep 30.

Authors

Sefater Gbashi¹, Patrick Berka Njobeh¹, Sarah De Saeger², Marthe De Boevre², Ntakadzeni Edwin Madala³

Affiliations

¹ Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, P.O Box 17011, Doornfontein Campus, 2028 Gauteng, South Africa.
² Centre of Excellence in Mycotoxicology and Public Health, Department of Bioanalysis, Ghent University, 9000 Ghent, Belgium.
³ Department of Biochemistry, School of Mathematical and Natural Sciences, University of Venda, Thohoyandou, South Africa.

PMID: 31648172
DOI: 10.1016/j.foodchem.2019.125526

Abstract

Effective management of mycotoxins rely on stringent regulation and routine surveillance of food/feed commodities via efficient analysis, hence the continuous need for improved methods. The present study developed, optimized and validated a modified pressurized hot water extraction (PHWE) method for the simultaneous extraction of multi-mycotoxins from maize and subsequent quantification on LC-MS/MS. The PHWE system was modified using ethanol (EtOH) as a cosolvent, while a numerical modelling approach, the central composite design (CCD), was adopted for the optimization of the extraction conditions. Using the optimized method, it was possible to effectively extract and quantify 15 different mycotoxins from maize in a single step with satisfactory linearities (0.986-0.999), recoveries (14-124%) and other associated method validation parameters. Further efficacious application of the method to real samples re-affirmed the prospects of PHWE as a suitable, cost-effective and greener alternative to traditional methods of mycotoxin extraction.

Keywords: Central composite design; Chemometric-assisted optimization; Mycotoxins; Pressurized hot water extraction.

MeSH terms

Chromatography, High Pressure Liquid
Hot Temperature
Limit of Detection
Mycotoxins / analysis*
Mycotoxins / isolation & purification
Pressure
Reproducibility of Results
Tandem Mass Spectrometry
Water / chemistry*
Zea mays / metabolism*

Substances

Mycotoxins
Water