Kinetic and solvent isotope effects in oxidation of halogen derivatives of tyramine catalyzed by monoamine oxidase A

J Biochem. 2020 Jan 1;167(1):49-54. doi: 10.1093/jb/mvz089.

Abstract

The isotope effects approach was used to elucidate the mechanism of oxidative deamination of 3'-halotyramines, catalyzed by monoamine oxidase A (EC 1.4.3.4). The numerical values of kinetic isotope effect (KIE) and solvent isotope effect (SIE) were established using a non-competitive spectrophotometric technique. Based upon KIE and SIE values, some of the mechanistic details of investigated reaction were discussed.

Keywords: 3′-halotyramines; MAO A; deuterium; enzyme mechanism; isotope effects.

MeSH terms

  • Biocatalysis
  • Fluorine Radioisotopes
  • Humans
  • Iodine Isotopes
  • Kinetics
  • Monoamine Oxidase / metabolism*
  • Oxidation-Reduction
  • Solvents / chemistry
  • Tyramine / metabolism*

Substances

  • Fluorine Radioisotopes
  • Iodine Isotopes
  • Solvents
  • Monoamine Oxidase
  • monoamine oxidase A, human
  • Tyramine