Degradation of phthalic acid esters (PAEs) by an enzyme mimic and its application in the degradation of intracellular DEHP

Xia Li; Jianpeng Li; Junxiang Zhu; Sijia Hao; Guozhen Fang; Jifeng Liu; Shuo Wang

doi:10.1039/c9cc06794a

Degradation of phthalic acid esters (PAEs) by an enzyme mimic and its application in the degradation of intracellular DEHP

Chem Commun (Camb). 2019 Nov 18;55(89):13458-13461. doi: 10.1039/c9cc06794a. Epub 2019 Oct 24.

Authors

Xia Li¹, Jianpeng Li², Junxiang Zhu³, Sijia Hao¹, Guozhen Fang¹, Jifeng Liu¹, Shuo Wang⁴

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, P. R. China. liujifeng111@gmail.com s.wang@tust.edu.cn.
² School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan, Shandong 250353, P. R. China.
³ College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, P. R. China.
⁴ State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, P. R. China. liujifeng111@gmail.com s.wang@tust.edu.cn and Research Center of Food Science and Human Health, School of Medicine, Nankai University, Tianjin 300071, P. R. China.

PMID: 31647069
DOI: 10.1039/c9cc06794a

Abstract

Here, an enzyme mimic inspired by serine proteases was developed for the degradation of PAEs. This enzyme mimic, comprised an active site (SHD), a self-assembling polypeptide (LKLKLKL), a spacer (GGG) and a polymerizable site (DOPA) and showed high activity towards the degradation of DEHP in cells.

MeSH terms

Cell Survival / drug effects
Diethylhexyl Phthalate / chemistry
Diethylhexyl Phthalate / metabolism*
Diethylhexyl Phthalate / pharmacology
Esters / chemistry
Esters / metabolism*
Esters / pharmacology
HeLa Cells
Humans
Hydrogen-Ion Concentration
Particle Size
Phthalic Acids / chemistry
Phthalic Acids / metabolism*
Phthalic Acids / pharmacology
Serine Proteases / chemistry
Serine Proteases / metabolism*
Surface Properties
Temperature

Substances

Esters
Phthalic Acids
Diethylhexyl Phthalate
Serine Proteases