Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.
Keywords: ATP-gated channels; Double-electrode patch clamp; Electrophysiology; Oocytes; P2X; Receptor function; Surface trafficking; Xenopus.