P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes

Methods Mol Biol. 2020:2041:243-259. doi: 10.1007/978-1-4939-9717-6_18.

Abstract

Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.

Keywords: ATP-gated channels; Double-electrode patch clamp; Electrophysiology; Oocytes; P2X; Receptor function; Surface trafficking; Xenopus.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Biotinylation / methods*
  • Blotting, Western
  • Cell Membrane / metabolism*
  • Cell Movement
  • Electrophysiology / methods*
  • Ion Channel Gating
  • Oocytes / cytology
  • Oocytes / physiology*
  • Patch-Clamp Techniques / methods
  • Receptors, Purinergic P2X / physiology*
  • Xenopus laevis / physiology*

Substances

  • Receptors, Purinergic P2X
  • Adenosine Triphosphate