Multimeric Purinoceptor Detection by Bioluminescence Resonance Energy Transfer

Vincent Compan; François Rassendren

doi:10.1007/978-1-4939-9717-6_11

Multimeric Purinoceptor Detection by Bioluminescence Resonance Energy Transfer

Methods Mol Biol. 2020:2041:155-162. doi: 10.1007/978-1-4939-9717-6_11.

Authors

Vincent Compan^{1

2}, François Rassendren^{3

4}

Affiliations

¹ IGF, University of Montpellier, CNRS, INSERM, Montpellier, France. vincent.compan@igf.cnrs.fr.
² Labex ICST, Montpellier, France. vincent.compan@igf.cnrs.fr.
³ IGF, University of Montpellier, CNRS, INSERM, Montpellier, France.
⁴ Labex ICST, Montpellier, France.

PMID: 31646487
DOI: 10.1007/978-1-4939-9717-6_11

Abstract

Assays based on bioluminescence resonance energy transfer (BRET) provide a sensitive and simple method to study protein-protein interactions in live cells. Here we describe a protocol using BRET technique to investigate potential interactions between P2X subunits. This approach combined with bimolecular fluorescence complementation (BiFC) can also be employed to determine the stoichiometry of heteromeric P2X receptors.

Keywords: BRET; BiFC; Bioluminescence; Heteromer; P2X; Stoichiometry.

MeSH terms

Bioluminescence Resonance Energy Transfer Techniques / methods*
Humans
Luminescent Measurements
Protein Binding
Protein Interaction Mapping / methods*
Protein Multimerization*
Receptors, Purinergic P2X / chemistry*
Receptors, Purinergic P2X / metabolism*
Signal Transduction

Substances

Receptors, Purinergic P2X