Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole-Mount In Situ Hybridization in Xenopus Embryos

Methods Mol Biol. 2020:2041:87-106. doi: 10.1007/978-1-4939-9717-6_6.

Abstract

Xenopus embryos are one of the most used animal models in developmental biology and are well suited for apprehending functions of signaling pathways during embryogenesis. To do so, it is necessary to be able to detect expression pattern of the key genes of these signaling pathways. Here we describe the whole-mount in situ hybridization technique to investigate the expression pattern of ectonucleotidases and purinergic receptors during embryonic development.

Keywords: Ectonucleotidases; Expression pattern; P2X receptors; Purinergic signaling pathway; Whole-mount in situ hybridization; Xenopus embryo.

MeSH terms

  • 5'-Nucleotidase / genetics
  • 5'-Nucleotidase / metabolism*
  • Animals
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / metabolism*
  • Embryonic Development
  • Female
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization / methods*
  • RNA Probes
  • Receptors, Purinergic / genetics
  • Receptors, Purinergic / metabolism*
  • Signal Transduction
  • Xenopus Proteins / genetics
  • Xenopus Proteins / metabolism*
  • Xenopus laevis / embryology
  • Xenopus laevis / metabolism*

Substances

  • RNA Probes
  • Receptors, Purinergic
  • Xenopus Proteins
  • 5'-Nucleotidase