Using RNA Interference for Purinoceptor Knockdown In Vivo

Rebeca Padrão Amorim; Iscia Teresinha Lopes Cendes; Maria Jose da Silva Fernandes

doi:10.1007/978-1-4939-9717-6_5

Using RNA Interference for Purinoceptor Knockdown In Vivo

Methods Mol Biol. 2020:2041:77-86. doi: 10.1007/978-1-4939-9717-6_5.

Authors

Rebeca Padrão Amorim¹, Iscia Teresinha Lopes Cendes², Maria Jose da Silva Fernandes^{3

4}

Affiliations

¹ Departamento de Neurologia e Neurocirurgia, Disciplina de Neurociência, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil.
² Departamento de Genetica Médica, Escola de Ciências Médicas da Universidade de Campinas-UNICAMP, Campinas, São Paulo, Brazil.
³ Departamento de Neurologia e Neurocirurgia, Disciplina de Neurociência, Universidade Federal de São Paulo-UNIFESP, São Paulo, Brazil. mjsfernandes19@unifesp.br.
⁴ Escola Paulista de Medicina, Universidade Federal de SÐo Paulo (EPM/UNIFESP), São Paulo, Brazil. mjsfernandes19@unifesp.br.

PMID: 31646481
DOI: 10.1007/978-1-4939-9717-6_5

Abstract

RNA interference (RNAi) is a powerful post-transcriptional gene silencing (PTGS) induced by small double-stranded RNA (dsRNA). The method allows silencing of genes of interest by translation inhibition or by mRNA degradation. In this chapter, we provide a brief overview of the mechanisms involved in each step of gene silencing. A nonviral infusion of short siRNA into ventricular system of rats was used to study purinoceptor in the rat brain.

Keywords: Brain; Hippocampus; Purinergic receptor; RNAi; Rat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Gene Silencing*
RNA Interference*
RNA, Small Interfering / genetics*
Rats
Receptors, Purinergic / chemistry*
Receptors, Purinergic / genetics
Signal Transduction

Substances

RNA, Small Interfering
Receptors, Purinergic