Biocompatible synthesis of reduced graphene oxide from Euphorbia heterophylla (L.) and their in-vitro cytotoxicity against human cancer cell lines

K Lingaraju; H Raja Naika; G Nagaraju; H Nagabhushana

doi:10.1016/j.btre.2019.e00376

Biocompatible synthesis of reduced graphene oxide from Euphorbia heterophylla (L.) and their in-vitro cytotoxicity against human cancer cell lines

Biotechnol Rep (Amst). 2019 Sep 12:24:e00376. doi: 10.1016/j.btre.2019.e00376. eCollection 2019 Dec.

Authors

K Lingaraju¹, H Raja Naika¹, G Nagaraju², H Nagabhushana³

Affiliations

¹ Department of Studies and Research in Biotechnology, Tumkur University, Tumakuru, 572103, India.
² Department of Chemistry, Siddaganga Institute of Technology, Tumkur University, Tumakuru, 572103, India.
³ Prof. C.N.R. Rao Centre for Advanced Material Science, Tumkur University, Tumakuru, 572103, India.

Abstract

Facile and biocompatible synthesis of reduced graphene oxide from graphene oxide as a precursor and aqueous leaves extract of Euphorbia heterophylla (L.), act as a reducing /capping /stabilizing agent by green chemistry approaches. The obtained product was analyzed by Ultraviolet-Visible spectroscopy (UV-vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and Scanning electron Microscopy (SEM). In addition to this, the significant cytotoxicity of rGO studied against cancerous cell lines such as A549- Human Lung cancer cell line and HepG2-Human Hepatocarcinoma Cell lines in-vitro. These results indicate that the biocompatible synthesis of rGO is straightforward, inexpensive and environmentally friendly for promising large-scale production of industrial purpose and then finding further biomedical applications.

Keywords: Cytotoxicity; Euphorbia heterophylla (L.); Reduced graphene oxide (rGO); X-ray diffraction.