New azaphilones from Penicillium variabile, a fungal endophyte from roots of Aconitum vilmorinianum

J Antibiot (Tokyo). 2020 Feb;73(2):77-81. doi: 10.1038/s41429-019-0250-4. Epub 2019 Oct 21.

Abstract

Two new azaphilone derivatives, comazaphilones G and H (1 and 2), together with eight known analogues (3-10), were isolated from an endophytic fungus Penicillium variabile. Their structures were established on the basis of extensive spectroscopic analysis. Compounds 1, 2 and 4-10 were tested their nitric oxide inhibitory activities in lipopolysaccharide-activated RAW 264.7 macrophage cells. Compounds 1, 2 and 4-9 showed significant nitric oxide inhibitory activities with IC50 values ranged from 4.35 ± 0.05 to 40.52 ± 0.47 μM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aconitum / microbiology*
  • Animals
  • Benzopyrans / chemistry
  • Benzopyrans / isolation & purification*
  • Benzopyrans / pharmacology
  • Endophytes / chemistry
  • Inhibitory Concentration 50
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mice
  • Nitric Oxide / antagonists & inhibitors*
  • Penicillium / chemistry*
  • Pigments, Biological / chemistry
  • Pigments, Biological / isolation & purification*
  • Pigments, Biological / pharmacology
  • Plant Roots
  • RAW 264.7 Cells

Substances

  • Benzopyrans
  • Lipopolysaccharides
  • Pigments, Biological
  • azaphilone
  • Nitric Oxide