High-throughput sequencing analysis of marine pioneer surface-biofilm bacteria communities on different PDMS-based coatings

Abstract

Marine biofilms, the attachment of marine microorganisms on artificial surfaces in natural seawater, play critical roles in the development of marine biofouling, which pave ways for the settlement and colonization of sessile invertebrate larvae. Despite the excellent microbe-inhibitory effect of polydimethylsiloxane (PDMS)-based coatings, marine bacteria could still attach to surfaces and form natural biofilms. However, there is little information available on the common structural features of pioneer surface-biofilm bacteria (PSB) communities on different PDMS-based coatings with regard to their compositions, distributions and diversity. Herein, the present study aims to explore the compositional and structural features of the PSB communities on different PDMS-based coatings using 16S rRNA gene amplicon sequencing in terms of the taxonomic structures at phylum, family and genus level. The results revealed the PSB communities on different PDMS-based coatings possessed high similarities in compositional, structural and diversity features, but varied greatly in relative abundance and distributions. Proteobacteria was the most diverse and overwhelming phylum in biofilms formed on all PDMS-based coatings, followed by Cyanobacteria. In addition, the decreased abundance of Proteobacteria and the increased abundance of Cyanobacteria on the carbon nanotubes (CNTs)-modifed PDMS composites (CPCs) may contribute to their differential anti-biofouling effect against the colonization of juvenile macrofoulers.

Keywords: Carbon nanotubes; High-throughput sequencing; Marine biofouling; Pioneer surface-biofilm bacteria; Polydimethylsiloxane.