Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

Go-Woon Choi; June-Pyo Oh; In-Sook Cho; Hye-Kyoung Ju; Wen-Xing Hu; Boram Kim; Eun-Young Seo; Jong-Seok Park; Leslie L Domier; John Hammond; Kihak Song; Hyoun-Sub Lim

doi:10.5423/PPJ.NT.12.2018.0286

Full-Length Infectious Clones of Two New Isolates of Tomato Mosaic Virus Induce Distinct Symptoms Associated with Two Differential Amino Acid Residues in 128-kDa Protein

Plant Pathol J. 2019 Oct;35(5):538-542. doi: 10.5423/PPJ.NT.12.2018.0286. Epub 2019 Oct 1.

Authors

Affiliations

¹ Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
² Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA, Wanju 55365, Korea.
³ Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
⁴ Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
⁵ United States Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA.
⁶ Department of Urology, Chungnam National University School of Medicine, Daejeon 34134, Korea.

Abstract

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

Keywords: difference of infectivity; full-length infectious cDNA clone; tobamovirus; tomato mosaic virus (ToMV).