In the present study, the performance of the liquid nitrogen frozen and thinned bovine pericardium was studied and compared with the porcine pericardium. The microstructure and mechanical properties of the bovine pericardium were observed and tested by hematoxylin-eosin (HE) staining and tensile test respectively. In all conditions, porcine pericardium was selected as a control group. The results showed that there was little difference in the performance of bovine pericardium after being frozen by liquid nitrogen. The secant modulus and ultimate strength of the thinned bovine pericardium were similar to those of porcine pericardium, however, the elastic modulus was a little higher than porcine pericardium. The study suggested that the performance of the thinned bovine pericardium was similar to those of porcine pericardium. It was easy for the thinned bovine pericardium to obtain a relatively ideal thickness and expected performance, therefore, the thinned bovine pericardium can be used as the materials of transcatheter aortic valve leaflets.
本文研究了牛心包液氮冷冻并减薄后的性能变化，并与猪心包进行了对比。采用苏木精-伊红（HE）染色的方法进行组织学微结构观察，并通过拉伸试验评价了冷冻和减薄对心包力学性能的影响，组织学观察和力学性能评价均采用猪心包作为对照。结果表明牛心包在液氮冷冻后性能未见区别；冷冻减薄后，割线模量和极限强度与猪心包基本相当，弹性模量略高于猪心包。研究表明，牛心包减薄容易获得理想的厚度和预期的性能，其可作为经导管瓣膜瓣叶材料的一种选择。.
Keywords: bovine pericardium; liquid nitrogen freezing; thinning; transcatheter aortic valve.