Screening, identification, and low-energy ion modified breeding of a yeast strain producing high level of 3-hydroxypropionic acid

Abstract

3-Hydroxypropionic acid (3HP) is an important platform chemical with a wide range of applications. The biological preparation of this compound is safe and low cost. In this study, orchard soil and human waste were used as raw materials to screen microbial strains that could produce 3HP in selective medium containing varying amounts of propionic acid. A yeast strain that can use propionic acid as substrate and produce 48.96 g/L 3HP was screened. Morphological observation, physiological and biochemical identification, and 26s rDNA sequencing identified the IS451 strain as Debaryomyces hansenii. The low-energy ion N⁺ , with the energy of 10 keV and a dose of 70 × 2.6 × 10¹³ ions/cm² , was implanted into the IS451 strain. The mutant strain WT39, whose 3HP titer reached 62.42 g/L, was obtained. The strain exhibited genetic stability and tolerance to high concentrations of propionic acid and was considered to have broad application prospects.

Keywords: 3-Hydroxypropionic acid (3HP); low-energy ion; modification; mutagenesis; yeast.