Differential expression of activin βA and βB genes in female allotriploid and diploid red crucian carp Carassius auratus red var

Abstract

activin β_A and β_B from diploid and allotriploid crucian carp were cloned.The differential expression of activin β_A and β_B genes in female allotriploid and diploid red crucian carp Carassius auratus red var. were studied and found to be expressed in all the tested tissues; particularly, the expression of activin β_A and β_B was elevated in the ovaries of allotriploids and differential expression in pituitaries during the non-breeding season and the breeding season period. The immunohistochemistry indicated that the abnormal triploid ovaries were dominated by small oogonium-like cells with dense signals and that the elevated expression of activin β_A and β_B in the ovaries of allotriploids may be related to allotriploid sterility.

Keywords: Carassius auratus red. var; activin βA; activin βB; allotriploid; differential expression; sterility.