ITLN in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var ♂) is involved in host defense against bacterial infection

Dev Comp Immunol. 2020 Feb:103:103520. doi: 10.1016/j.dci.2019.103520. Epub 2019 Oct 15.

Abstract

Hybrid genotypes in fish may be less susceptible to pathogenic infection. ITLN, a novel lectin, not only exhibits a regulatory role in pathogenic infection, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-ITLN was 945 bp and encoded 314 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-ITLN was observed in liver. Aeromonas hydrophila challenge can sharply increased WR-ITLN mRNA expression in liver, kidney and spleen. The purified WR-ITLN protein can directly bind to A. hydrophila and S. agalactiae, reduce their relative bacterial activity and limit bacterial growth in vitro in the presence of Ca2+. In addition, the treatment of WR-ITLN + Ca2+ can restrict bacterial dissemination in vivo and attenuate production of pro-inflammatory cytokines. These results indicated that WR-ITLN can confer protection against bacteria-stimulated MyD88-dependent pro-inflammatory cytokines activation in a Ca2+-dependent manner.

Keywords: Aeromonas hydrophila; Diploid hybrid fish; Gene expression; ITLN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas hydrophila / immunology
  • Animals
  • Chimera / genetics
  • Chimera / immunology*
  • Diploidy
  • Female
  • Fish Diseases / immunology*
  • Fish Proteins / genetics
  • Fish Proteins / immunology*
  • Galectins / genetics
  • Galectins / immunology*
  • Genotype
  • Goldfish / genetics
  • Goldfish / immunology*
  • Gram-Negative Bacterial Infections / immunology
  • Gram-Negative Bacterial Infections / veterinary*
  • Male

Substances

  • Fish Proteins
  • Galectins