We used shell-vial assay with a medium that buffered rifampin to isolate routine culture-resistant Mycobacterium tuberculosis bacteria from cerebrospinal fluid and rifampin-containing intervertebral disc and vertebral corpus of a patient in treatment for Pott's disease and disseminated tuberculosis. Whole-genome sequencing confirmed M. tuberculosis lineage 4 (Euro-American) strain.
Keywords: France; Mycobacterium tuberculosis; antimicrobial resistance; bacteria; culture; shell-vial assay; tuberculosis; tuberculosis and other mycobacteria.