Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning

Kathryn M Frietze; Susan B Core; Alexandria Linville; Bryce Chackerian; David S Peabody

doi:10.1007/978-1-4939-9853-1_9

Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning

Methods Mol Biol. 2020:2070:157-171. doi: 10.1007/978-1-4939-9853-1_9.

Authors

Kathryn M Frietze^{1

2}, Susan B Core³, Alexandria Linville³, Bryce Chackerian³, David S Peabody³

Affiliations

¹ Department of Molecular Genetics and Microbiology, School of Medicine, University of New Mexico Health Sciences, Albuquerque, NM, USA. kfrietze@salud.unm.edu.
² Clinical and Translational Science Center, University of New Mexico Health Sciences, Albuquerque, NM, USA. kfrietze@salud.unm.edu.
³ Department of Molecular Genetics and Microbiology, School of Medicine, University of New Mexico Health Sciences, Albuquerque, NM, USA.

PMID: 31625095
DOI: 10.1007/978-1-4939-9853-1_9

Abstract

Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for the broad assessment of selectant populations. Here, we describe a method for using a phage-display platform to assess antibody specificity in human serum. We describe the method with reference to the bacteriophage MS2 virus-like particle (VLP) platform, but it can be adapted to other phage-display technologies as well.

Keywords: Affinity selection; Antibodies; Deep sequencing; Pathogens; Phage display; Serum; Virus-like particle.

MeSH terms

Antibody Specificity*
Epitopes / chemistry*
Humans
Levivirus* / chemistry
Levivirus* / genetics
Peptide Library*
Serum / chemistry*
Single-Chain Antibodies* / chemistry
Single-Chain Antibodies* / genetics

Substances

Epitopes
Peptide Library
Single-Chain Antibodies