Expression and predictive value of miR-489 and miR-21 in melanoma metastasis

Hao Mo; Jian Guan; Zhen-Chao Yuan; Xiang Lin; Zhen-Jie Wu; Bin Liu; Ju-Liang He

doi:10.12998/wjcc.v7.i19.2930

Expression and predictive value of miR-489 and miR-21 in melanoma metastasis

World J Clin Cases. 2019 Oct 6;7(19):2930-2941. doi: 10.12998/wjcc.v7.i19.2930.

Authors

Hao Mo¹, Jian Guan¹, Zhen-Chao Yuan¹, Xiang Lin¹, Zhen-Jie Wu¹, Bin Liu¹, Ju-Liang He²

Affiliations

¹ Department of Bone and Soft Tissue Surgery, Guangxi Medical University Cancer Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
² Department of Bone and Soft Tissue Surgery, Guangxi Medical University Cancer Hospital, Nanning 530021, Guangxi Zhuang Autonomous Region, China. hejuliang0@qq.com.

Abstract

Background: Melanoma is a highly malignant skin tumour, and is one of the most rapidly growing malignant tumors in recent years. According to statistics, the morbidity of cancer increases with age, accounting for 1.6% of new cancer cases and 0.6% of deaths worldwide. Melanoma has a serious impact on society and families, thus it is of great significance to find biological markers related to the diagnosis and treatment of melanoma.

Aim: To explore the expression and predictive value of mir-489 and mir-21 in melanoma metastasis.

Methods: A total of 60 patients with malignant melanoma treated at our hospital from June 2017 to December 2018 were selected as a research group, while 40 healthy subjects were selected as a control group. qRT-PCR technique was used to detect miR-489 and miR-21 in serum of the two groups. ROC curve was drawn to evaluate the predictive value and diagnostic efficiency. Spearman test was used for correlation analysis. Logistic single- and multiple-factor analyses were performed to identify the risk factors related to melanoma metastasis.

Results: The expression of miR-489 in the research group was significantly lower than that in the control group (P < 0.001). However, the expression of miR-21 in the research group was significantly higher than that in the control group (P < 0.001). The expression of miR-489 and miR-21 was related to TNM stage and metastasis (P < 0.001). In the diagnosis of melanoma patients, the sensitivity, specificity, and AUC of miR-489 alone were 75.56%, 80.00%, and 0.852, respectively. The sensitivity, specificity, and AUC of miR-21 alone were 77.78%, 82.22%, and 0.844, respectively. MiR-489 was negatively correlated with TNM stage of melanoma (r = -0.612, P < 0.001), while miR-21 was positively correlated with TNM stage (r = 0.609, P < 0.001). Logistic single- and multiple-factor regression analyses showed that TNM stage, miR-489, and mir-21 were independent risk factors for malignant melanoma metastasis.

Conclusion: MiR-489 and miR-21 may participate in the process of melanoma occurrence, development, and metastasis, and can be used as potential serum biomarkers for melanoma metastasis diagnosis and disease assessment.

Keywords: Melanoma metastasis; MiR-21; MiR-489; Predictive value.