Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

Wei-Tao Dou; Li-Fang Liu; Jie Gao; Yi Zang; Guo-Rong Chen; Robert A Field; Tony D James; Jia Li; Xiao-Peng He

doi:10.1039/c9cc06399d

Fluorescence imaging of a potential diagnostic biomarker for breast cancer cells using a peptide-functionalized fluorogenic 2D material

Chem Commun (Camb). 2019 Oct 31;55(88):13235-13238. doi: 10.1039/c9cc06399d.

Authors

Wei-Tao Dou¹, Li-Fang Liu², Jie Gao², Yi Zang³, Guo-Rong Chen¹, Robert A Field⁴, Tony D James⁵, Jia Li³, Xiao-Peng He¹

Affiliations

¹ Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. xphe@ecust.edu.cn.
² Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China. xphe@ecust.edu.cn and National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd, Shanghai 201203, P. R. China. yzang@simm.ac.cn jli@simm.ac.cn.
³ National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 189 Guo Shoujing Rd, Shanghai 201203, P. R. China. yzang@simm.ac.cn jli@simm.ac.cn.
⁴ Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
⁵ Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.

PMID: 31621698
DOI: 10.1039/c9cc06399d

Abstract

Protein C receptor (PROCR) is a recently discovered transmembrane biomarker for several tissue stem cells and is highly expressed in triple-negative breast cancer (TNBC) patient-derived xenografts. Herein, to enrich the toolbox for the biochemical evaluation of PROCR, we have developed a peptide-functionalized fluorogenic 2D material based on the self-assembly between a fluorescent peptide probe and thin-layer molybdenum disulfide. The material developed was suitable for the sensitive detection of PROCR recombinant protein in buffer solution and the fluorescence imaging of TNBC cells that express high levels of PROCR.

MeSH terms

Biomarkers, Tumor / analysis*
Cell Line, Tumor
Endothelial Protein C Receptor / analysis*
Female
Fluorescent Dyes / chemistry*
Humans
MCF-7 Cells
Molecular Structure
Optical Imaging*
Particle Size
Peptides / chemistry*
Spectrometry, Fluorescence
Surface Properties
Triple Negative Breast Neoplasms / diagnostic imaging*

Substances

Biomarkers, Tumor
Endothelial Protein C Receptor
Fluorescent Dyes
Peptides