High-Frequency Raman Analysis in Biological Tissues Using Dual-Wavelength Excitation Raman Spectroscopy

Appl Spectrosc. 2020 Feb;74(2):241-244. doi: 10.1177/0003702819881762. Epub 2019 Nov 7.

Abstract

A dual-wavelength excitation Raman probe with laser inputs at 866 nm or 1064 nm is customized and integrated into a compact Raman spectrometer that is based on an InGaAs detector. Under 1064 nm illumination, the spectrometer detects fingerprint Raman signals below 2000 cm-1. While under 866 nm illumination, the spectral range is extended to cover high-frequency region (2400-4000 cm-1) that includes major C-H and O-H Raman vibrations. We demonstrate that the dual excitation InGaAs Raman is beneficial in detecting high-frequency Raman signals, especially water contents in high-fluorescent biological samples such as human dental tissues, grape skin, and plum skin due to the suppressed fluorescence interference.

Keywords: InGaAs; Raman spectroscopy; dual-wavelength excitation; high-frequency Raman; hydration probing.

MeSH terms

  • Dental Enamel / chemistry*
  • Fluorescence
  • Fruit / chemistry*
  • Humans
  • Prunus domestica / chemistry
  • Spectrum Analysis, Raman / methods*
  • Vitis / chemistry
  • Water / chemistry*

Substances

  • Water