The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1

Methods Mol Biol. 2020:2060:409-418. doi: 10.1007/978-1-4939-9814-2_25.

Abstract

Understanding how herpes simplex virus-1 (HSV-1) interacts with different parts of the neuron is fundamental in understanding the mechanisms behind HSV-1 transport during primary and recurrent infections. In this chapter, we describe a unique neuronal culture system that is capable of compartmentalizing neuronal cell bodies from their axons to study the transport of HSV-1 along axons. The ability to separate neuronal cell bodies and axons provides a unique model to investigate the mechanisms used by HSV-1 for viral transport, assembly, and exit from different parts of the neuron.

Keywords: Axons; Herpes simplex virus; Microfluidics; Neurons; Transport.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axonal Transport*
  • Axons* / metabolism
  • Axons* / pathology
  • Axons* / virology
  • Ganglia, Spinal* / metabolism
  • Ganglia, Spinal* / pathology
  • Ganglia, Spinal* / virology
  • Herpesvirus 1, Human / metabolism*
  • Lab-On-A-Chip Devices*
  • Microfluidic Analytical Techniques*
  • Rats
  • Rats, Wistar