N-Doped Graphene Oxide Decorated with PtCo Nanoparticles for Immobilization of Double-Stranded Deoxyribonucleic Acid and Investigation of Clenbuterol-Induced DNA Damage

Mian Hasnain Nawaz; Jianan Xu; Zhongqian Song; Sara Riaz; Dongxue Han; Li Niu

doi:10.1021/acsomega.9b02184

N-Doped Graphene Oxide Decorated with PtCo Nanoparticles for Immobilization of Double-Stranded Deoxyribonucleic Acid and Investigation of Clenbuterol-Induced DNA Damage

ACS Omega. 2019 Sep 26;4(15):16524-16530. doi: 10.1021/acsomega.9b02184. eCollection 2019 Oct 8.

Authors

Mian Hasnain Nawaz^{1

2

3}, Jianan Xu², Zhongqian Song², Sara Riaz³, Dongxue Han^{1

2}, Li Niu^{1

2}

Affiliations

¹ Center for Advanced Analytical Science, c/o School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, P.R. China.
² State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P.R. China.
³ Interdisciplinary Research Centre in Biomedical Materials (IRCBM) and Department of Chemistry, COMSATS University Islamabad, Lahore Campus, Lahore 54000, Pakistan.

Abstract

We demonstrate here a facile hydrothermal-assisted formation of PtCo alloy nanoparticles (NPs) and their simultaneous anchoring on the graphitic surface of N-doped graphene oxide (NGO). Doping induced nanopores in the carbon surface to facilitate the uniform and homogeneous anchoring of alloy nanoparticles. It was revealed that the formation of PtCo NPs on an NGO interface plodded excellent tendency toward double-stranded deoxyribonucleic acid (dsDNA). The dsDNA immobilization was enabled by the presence of several oxidation states of Pt and Co. The same property was further used to monitor the direct detection of dsDNA damage induced by clenbuterol via screen-printed carbon electrodes. Cyclic voltammetric and electrochemical impedance spectroscopic characterization traced well the dsDNA attachment on the modified electrode surface. Differential pulsed voltammetry was further used as a tool to monitor the characteristic guanine peak before and after incubating with clenbuterol used as a damage probe for the dsDNA. The findings can further be appurtenant in exploring dsDNA immobilization protocols and developing analytical methods for determination of various dsDNA damaging agents.