Intestinal Surgery Contributes to Acute Cerebellar Ataxia Through Gut Brain Axis

Jie Yu; Yuanming Fan; Li Wang; Yanjuan Huang; Jingyi Xia; Le Ding; Chun-Feng Wu; Xiaopeng Lu; Gaoxiang Ma; Samuel Kim; Guo Zheng; Hu Guo; Gang Zhang

doi:10.3389/fneur.2019.00995

Intestinal Surgery Contributes to Acute Cerebellar Ataxia Through Gut Brain Axis

Front Neurol. 2019 Sep 20:10:995. doi: 10.3389/fneur.2019.00995. eCollection 2019.

Authors

Jie Yu¹, Yuanming Fan², Li Wang¹, Yanjuan Huang¹, Jingyi Xia¹, Le Ding¹, Chun-Feng Wu¹, Xiaopeng Lu¹, Gaoxiang Ma², Samuel Kim³, Guo Zheng¹, Hu Guo¹, Gang Zhang¹

Affiliations

¹ Department of Neurology, Children's Hospital of Nanjing Medical University, Nanjing, China.
² Clinical Metabolomics Center, China Pharmaceutical University, Nanjing, China.
³ Department of Anesthesiology, Emory University School of Medicine, Atlanta, GA, United States.

Abstract

Introduction: Acute cerebellar ataxia (ACA) is the most common form of pediatric ataxia. Changes in gut flora can modulate the nervous system, influencing brain function via the gut-brain axis (GBA). This study aimed to illustrate the relationship between intestinal microbiota and ACA. Method: A total of 30 and 12 children were randomly sampled from history of intestinal surgery (HOIS) and no intestinal surgery groups (NHOIS), respectively. In addition, 10 healthy children who sought physical examination in Children's Hospital of Nanjing Medical University were recruited as a control group. The stool samples were 16S rRNA detected. Results: We observed that many ACA children had intestinal surgery history prior to the onset of ACA. The 16S rRNA sequencing indicated that HOIS and control groups were well-distinguished by principal component analysis. The discrepancy between HOIS and NHOIS groups were also displayed by principal component analysis score plot. However, no differences were found between NHOIS and control groups. The results of student's t-test were consistent with principal component analysis. A total of nine different genera were identified between HOIS and control groups. Five genera and a phylum showed significant differences between HOIS and NHOIS groups. Conclusion: Altered genera and phyla associated with ACA were identified. Our findings provide new insight into treating and preventing ACA.

Keywords: 16S rRNA; acute cerebellar ataxia; gut brain axis; gut flora; intestinal surgery contributes.