Objective: To evaluate the effects of intravenous injection of different blood components containing Babesia microti on B. microti infection in mice.
Methods: Healthy mice were infected with B. microti, and then blood samples were collected from the mouse orbit to prepare whole blood, serum-free blood components and pure red blood cells containing B. microti. Twenty seven BALB/c mice were divided into three groups, including the whole blood group, the serum-free blood component group and the pure red blood cell group, of 9 mice in each group, and then, each group was divided into three subgroups, of 3 mice in each subgroup, which were injected with 100 μL of blood components containing B. microti at concentrations of 9.00, 0.90, 0.09 B. microti parasites/μL (900, 90, 9 B. microti parasites) via the tail vein, respectively. Blood samples were collected from the mouse tail tip every other day since one day post-injection to prepare thin blood smears. Following Giemsa staining of blood smears, B. microti infection was identified in red blood cells using microscopy.
Results: Following injection of 900 B. microti parasites, B. microti was identified in the peripheral blood in the whole blood group and the serum-free blood component group 3 days post-injection, and the density of B. microti parasites started to increase 15 days post-injection and peaked 21 days post-injection, with 2.21% and 1.76% rates of B. microti infection in red blood cells, respectively. Subsequently, the density of B. microti parasites declined, and the percentage of B. microti infection in red blood cells tended to be 0 31 days post-injection. During the study period, no B. microti was found in the peripheral blood in the pure red blood cell group. Following injection of 90 B. microti parasites, B. microti was identified in the peripheral blood in the whole blood group 3 days post-injection, and the density of B. microti parasites increased 15 days post-injection and peaked 21 days post-injection, with a 1.35% rate of B. microti infection in red blood cells, while the percentage of B. microti infection in red blood cells tended to be 0 31 days post-injection. During the study period, no B. microti was detected in the peripheral blood in the serum-free blood component group or the pure red blood cell group. Following injection of 9 B. microti parasites, no B. microti was detected in the peripheral blood in the whole blood group, the serum-free blood component group or the pure red blood cell group.
Conclusions: Blood components and dose of B. microti parasites may affect intravenous injection of B. microti injection in mice, and transfusion of blood components may case a risk of Babesia infection.
[摘要] 目的 观察静脉注射含田鼠巴贝虫的不同成分血对小鼠巴贝虫感染的影响。方法 以田鼠巴贝虫感染健康小 鼠后眼眶采血, 制备染虫全血、去除血清的成分血及纯红细胞。将27只小鼠随机分为3组, 分别为全血组、无血清染虫 组、纯红细胞组, 每组9只; 每组设3个亚组, 每个亚组3只, 每只分别通过尾静脉注射100 μL浓度为9.00、0.90、0.09只/μL (分别含900、90、9只巴贝虫虫体) 的相应成分血。接种当天记为D0, 自D1起每隔1 d于小鼠尾尖采血涂薄血涂片, 吉氏 染色液染色后镜检观察各组小鼠红细胞染虫率。结果 注射900只田鼠巴贝虫后, 全血组、无血清组均于D3在外周血 中查见巴贝虫, 于D15虫密度开始升高, 并于D21虫密度达高峰, 红细胞染虫率分别为2.21%和1.76%; 随后虫密度下降, D31染虫率趋于0; 而纯红细胞组小鼠在观察期间未查见巴贝虫感染。注射90只田鼠巴贝虫, 仅全血组于D3在外周血查 见巴贝虫虫体, D15虫密度升高, D21虫血症达高峰, 红细胞染虫率为1.35%, D31染虫率趋于0; 无血清组和纯红细胞组 实验期间外周血中未查见巴贝虫。注射9只田鼠巴贝虫, 全血组、无血清组和纯红细胞组外周血中均未查见虫体。结 论 血液成分及感染虫数可能对小鼠静脉注射感染巴贝虫有一定影响, 输入成分血仍存在感染巴贝虫的风险。.
Keywords: Babesia microti; Blood component; Blood transfusion; Mouse.