A multichannel system integrating molecularly imprinted conductive polymers for ultrasensitive voltammetric determination of four steroid hormones in urine

Mikrochim Acta. 2019 Oct 14;186(11):695. doi: 10.1007/s00604-019-3797-7.

Abstract

This work reports on a modularized electrochemical method for the determination of the hormones cortisol, progesterone, testosterone and 17β-estradiol in urine. These hormones were employed as templates when generating molecular imprints from aniline and metanilic acid by electropolymerization on the surface of screen-printed electrodes. The electrically conductive imprint was characterized by SEM, AFM and cyclic voltammetry. A four-channel system was then established to enable simultaneous determination of the hormones by cyclic voltammetry. The detection limits for cortisol, progesterone, testosterone and 17β-estradiol are as low as 2, 2.5, 10 and 9 ag·mL-1 (for S/N = 3). Graphical abstract A four-channel system was established to enable simultaneous determination of 4 steroid hormones by cyclic voltammetry and by using moleculalry imprinted polymers.

Keywords: Electrochemical sensing; Hormones; Molecular imprinting; Multichannel potentiostat; Urine analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / chemistry
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Electrodes
  • Equipment Design
  • Estradiol / urine*
  • Humans
  • Hydrocortisone / urine*
  • Limit of Detection
  • Molecular Imprinting
  • Polymerization
  • Polymers / chemical synthesis
  • Polymers / chemistry*
  • Progesterone / urine*
  • Sulfanilic Acids / chemistry
  • Testosterone / urine*

Substances

  • Aniline Compounds
  • Polymers
  • Sulfanilic Acids
  • Testosterone
  • Progesterone
  • Estradiol
  • metanilic acid
  • aniline
  • Hydrocortisone