Objective: To investigate the effect of triptolide on the excursion of Tc and Th cells in peripheral blood of systemic lupus erythematosus (SLE) BALB/c-un nude mice induced by pristane.
Methods: Eighteen female BALB/c-un nude mice were randomly divided into blank, SLE and triptolide group, each with 6 mice by random table method. Group SLE and group triptolide were established by single intraperitoneal injection of pristane, and blank group was used as blank control group. SLE model was established by single intraperitoneal injection. Triptolide group was fed with triptolide at the dose of 5 mg/(kg·d), and the blank group and SLE group were fed normally. Blood samples were collected from the caudal vein before treatment and 1, 3 and 6 months after treatment respectively. Fluorescence labeled flow cytometry was used to delect Tc and Th lymphocyte subsets at different stages of treatment.
Results: After treatment for 3 and 6 moths, the percentages of Tcl, Thl cells and CD8+, Tcl/Tc2, Thl/Th2 and CD4+/CD8+ all decreased in the group of triptolide, and the percentage of CD4+, Tc2 and Th2 cells increased (P＜0.05).
Conclusion: The mechanism of triptolide in the treatment of SLE may be related with the excursion of Tc and Th cells to Tcl and Tc2 to maintain the relative homeostasis of Tc and Th cells at different stage, thus affecting the immune response and the inflammatory reaction.
题目: 雷公藤甲素对系统性红斑狼疮BALB/c-un裸小鼠外周血Tc与Th细胞漂移的影响.
目的: 观察雷公藤甲素对Pristane（姥鲛烷）诱发的系统性红斑狼疮（systemic lupus erythematosus，SLE）BALB/c-un裸小鼠外周血Tc与Th细胞漂移的影响。.
方法: 用随机表法将雌BALB/c-un裸小鼠18只机编号后随机均分为空白、SLE和雷公藤甲素3组，每组动物数6只。SLE组和雷公藤甲素组用Pristane单次腹腔注射的方法建立SLE模型，空白组作空白对照。雷公藤甲素组按 5 mg/（kg•d）灌服雷公藤甲，空白组和SLE组正常饲喂。分别于治疗前、治疗后1、3和6个月从尾静脉采血，采用荧光标记的流式细胞术检查治疗不同阶段Tc与Th淋巴细胞亚群。.
结果: 在治疗3和6个月时与SLE组同期比较，雷公藤甲素组Tcl、Thl细胞和CD8+百分比、Tcl/Tc2，Thl/Th2和CD4+/CD8+比值均降低，而CD4+、Tc2 和Th2细胞百分比均明显升高（P＜0.05）。.
结论: 雷公藤甲素治疗SLE的机制可能与其调节Tc与Th细胞向Tcl和Tc2漂移，维持治疗不同阶段Tc与Th细胞相对稳态而影响免疫应答及炎症反应过程有关。.