A merocyanine-based dual-mode optical probe for detection of hydrazine and its bioimaging application in vitro and vivo

Xiumei Guo; Shanshan Li; Shuai Mu; Yintang Zhang; Xiaoyan Liu; Haixia Zhang

doi:10.1016/j.saa.2019.117625

A merocyanine-based dual-mode optical probe for detection of hydrazine and its bioimaging application in vitro and vivo

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 5:226:117625. doi: 10.1016/j.saa.2019.117625. Epub 2019 Oct 7.

Authors

Xiumei Guo¹, Shanshan Li¹, Shuai Mu¹, Yintang Zhang², Xiaoyan Liu³, Haixia Zhang¹

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
² Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, China.
³ State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China. Electronic address: liuxiaoy@lzu.edu.cn.

PMID: 31605967
DOI: 10.1016/j.saa.2019.117625

Abstract

In this paper, a Merocyanine-based turn-on probe (McyA) has been developed for colorimetric and fluorescent dual-mode detection of N₂H₄ via an intra-molecular charge transfer (ICT) mechanism. In the presence of N₂H₄, the probe shows an obvious chromogenic response and fluorescent enhancement. Based on this feature, the synthesized McyA can be applied to quantify N₂H₄ concentration from 0.12 to 5.0 μM and 0.5-10 μM with a detection limit of 0.042 μM (1.3 ppb, S/N = 3) and 0.140 μM (4.5 ppb, S/N = 3), respectively. Moreover, McyA has also be successfully employed for imaging analysis of N₂H₄ distribution in different organs from the N₂H₄ ingested mice model, revealing the potential application of McyA as a powerful fluorescent sensor for tracking detection and risk assessment of hydrazine in vivo.

Keywords: Bioimaging; Dual-mode detection; Fluorescence probe; Hydrazine; In vivo.

MeSH terms

Animals
Benzopyrans / chemistry*
Benzopyrans / pharmacology
Biosensing Techniques / methods*
Cell Survival / drug effects
Colorimetry / methods
Fluorescent Dyes / chemistry*
Fluorescent Dyes / pharmacology
Hep G2 Cells
Humans
Hydrazines / analysis*
Indoles / chemistry*
Indoles / pharmacology
Limit of Detection
Materials Testing
Mice
Molecular Imaging / methods*
Spectrometry, Fluorescence / methods

Substances

Benzopyrans
Fluorescent Dyes
Hydrazines
Indoles
merocyanine
hydrazine