Directed evolution (DE) is a powerful approach for generating proteins with new chemical and physical properties. It mimics the principles of Darwinian evolution by imposing selective pressure on a large population of molecules harboring random genetic variation in DNA, such that sequences with specific desirable properties are generated and selected. We propose that combining DE and genome-editing (DE-GE) technologies represents a powerful tool to discover and integrate new traits into plants for agronomic and biotechnological gain. DE-GE has the potential to deliver a new green (r)evolution research platform that can provide novel solutions to major trait delivery aspirations for sustainable agriculture, climate-resilient crops, and improved food security and nutritional quality.
Keywords: CRISPR; directed evolution; food quality; genome editing; photosynthesis; productivity; resilience to stress.
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