Novel reassortant of H1N1 swine influenza virus detected in pig population in Russia

Ivan Sobolev; Olga Kurskaya; Sergey Leonov; Marsel Kabilov; Tatyana Alikina; Alexander Alekseev; Yuriy Yushkov; Takehiko Saito; Yuko Uchida; Junki Mine; Alexander Shestopalov; Kirill Sharshov

doi:10.1080/22221751.2019.1673136

Novel reassortant of H1N1 swine influenza virus detected in pig population in Russia

Emerg Microbes Infect. 2019;8(1):1456-1464. doi: 10.1080/22221751.2019.1673136.

Authors

Affiliations

¹ Department of Experimental Modeling and Pathogenesis of Infectious Diseases, Federal Research Center of Fundamental and Translational Medicine , Novosibirsk , Russia.
² Siberian Federal Scientific Centre of Agro- BioTechnologies , Krasnoobsk , Russia.
³ Institute of Chemical Biology and Fundamental Medicine , Novosibirsk , Russia.
⁴ Division of Transboundary Animal Disease, National Institute of Animal Health , Tsukuba , Japan.

Abstract

Pigs play an important role in interspecies transmission of the influenza virus, particularly as "mixing vessels" for reassortment. Two influenza A/H1N1 virus strains, A/swine/Siberia/1sw/2016 and A/swine/Siberia/4sw/2017, were isolated during a surveillance of pigs from private farms in Russia from 2016 to 2017. There was a 10% identity difference between the HA and NA nucleotide sequences of isolated strains and the most phylogenetically related sequences (human influenza viruses of 1980s). Simultaneously, genome segments encoding internal proteins were found to be phylogenetically related to the A/H1N1pdm09 influenza virus. In addition, two amino acids (129-130) were deleted in the HA of A/swine/Siberia/4sw/2017 compared to that of A/swine/Siberia/1sw/2016 HA.

Keywords: H1N1; Swine; influenza; phylogeny; reassortant.

MeSH terms

Animals
Genome, Viral
Influenza A Virus, H1N1 Subtype / classification
Influenza A Virus, H1N1 Subtype / genetics*
Influenza A Virus, H1N1 Subtype / isolation & purification
Orthomyxoviridae Infections / epidemiology
Orthomyxoviridae Infections / veterinary*
Phylogeny
Reassortant Viruses / genetics*
Reassortant Viruses / isolation & purification
Russia / epidemiology
Swine / microbiology*
Swine Diseases / epidemiology*
Swine Diseases / virology

Grants and funding

This work was supported by Russian Science Foundation: [Grant Number 17-44-07001]; Russian State funded budget project: [Grant Number АААА-А17-117020210021-7].