SpBAG1 promotes the WSSV infection by inhibiting apoptosis in mud crab (Scylla paramamosain)

Xiaomeng Ma; Xixiang Tang; Shanmeng Lin; Yi Gong; Ngoc Tuan Tran; Huaiping Zheng; Hongyu Ma; Jude Juventus Aweya; Yueling Zhang; Shengkang Li

doi:10.1016/j.fsi.2019.10.013

SpBAG1 promotes the WSSV infection by inhibiting apoptosis in mud crab (Scylla paramamosain)

Fish Shellfish Immunol. 2019 Nov:94:852-860. doi: 10.1016/j.fsi.2019.10.013. Epub 2019 Oct 7.

Authors

Affiliations

¹ Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Marine Biology Institute, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China.
² Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.
³ Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Marine Biology Institute, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. Electronic address: lisk@stu.edu.cn.

PMID: 31600594
DOI: 10.1016/j.fsi.2019.10.013

Abstract

Bcl-2 associated athanogene-1 (BAG1) is involved in various signalling pathways including apoptosis, cell proliferation, gene transcriptional regulation and signal transduction in animals. However the functions of BAG1 during the antiviral response of mud crab Scylla paramamosain is still unclear. In this study, the mud crab BAG1 (SpBAG1) was characterized to consist of 1761 nucleotides, containing an opening frame of 630bp encoding 209 amino acids with an ubiquitin domain and a BAG1 domain. SpBAG1 was found to be significantly up-regulated at 6 h-24 h, but down-regulated from 48 h-72 h in the hemocytes of mud crab after challenge with white spot syndrome virus (WSSV). RNAi knock-down of SpBAG1 significantly reduced the copies of WSSV and increased the apoptotic rate in mud crabs. The finding from this study suggested that SpBAG1 could promote the WSSV infection by inhibiting apoptosis in mud crab. Therefore, to the best of our knowledge, this is the first study demonstrating the role of SpBAG1 as a novel apoptosis inhibitor to promote virus infection in mud crab.

Keywords: Apoptosis; Mud crab; SpBAG1; WSSV.

MeSH terms

Amino Acid Sequence
Animals
Apoptosis
Arthropod Proteins / chemistry
Arthropod Proteins / genetics
Arthropod Proteins / immunology
Base Sequence
Brachyura / genetics*
Brachyura / immunology*
Gene Expression Profiling
Gene Expression Regulation / immunology*
Immunity, Innate / genetics*
Phylogeny
White spot syndrome virus 1 / physiology
bcl-Associated Death Protein / chemistry
bcl-Associated Death Protein / genetics*
bcl-Associated Death Protein / immunology*

Substances

Arthropod Proteins
bcl-Associated Death Protein